Virus:Article Title: Aurora-A Breaks Symmetry in Contractile Actomyosin Networks Independently of Its Role in Centrosome Maturation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-PAR-6 A gift from Yen Wei Lim (Temasek Lifesciences Laboratory) N/A Donkey anti-rabbit coupled to Cy5 Jackson ImmunoResearch 711-175-152; RRID: AB-2737252 Bacterial and Virus Strains E. coli: HT115(DE3): F-, mcrA, mcrB, IN(rrnD-rrnE)1, rnc14::Tn10(DE3 lysogen: lavUV5 promoter -T7 polymerase) (IPTG-inducible T7 polymerase) (RNAse III minus). .. Caenorhabditis Genetics Center WB-Strain: HT115(DE3) E. coli: OP50: E. coli B, uracil auxotroph Caenorhabditis Genetics Center WB-Strain: OP50 Chemicals, Peptides, and Recombinant Proteins Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# P1585 Natural auxin indole 3-acetic acid (IAA) A gift from Xu Jian (National University of Singapore)
Plasmid Preparation:Article Title: The Serotonergic System Tracks the Outcomes of Actions to Mediate Short-Term Motor Learning.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-5-HT rabbit polyclonal antibody Sigma-Aldrich S5545; RRID: AB_477522 anti-rabbit secondary antibody conjugated with Cy3 Jackson ImmunoResearch 711-165-152; RRID: AB_2307443 anti-rabbit secondary antibody conjugated with Cy5 Jackson ImmunoResearch 711-175-152; RRID: AB_2340607 Alexa Fluor 594 hydrazide, sodium salt Thermo Fisher Scientific A10438 Chemicals for Pharmacology Assays, Paralysis, and Anesthesia Hoechst 33342 Life Technologies H3570 Alpha-Bungarotoxin Life Technologies B1601 Tricaine Sigma-Aldrich A5040 Para-chlorophenylalanine (pCPA) Tocris Bioscience 0938 Metronidazole MP Biomedicals 02155710 DNA Materials epNTR-TagRFP Tabor et al., 2014 N/A tph2 promoter in Tol2 vector Yokogawa et al., 2012 N/A Transgenic Animals Tg(elavl3:H2B-GCaMP6f)jf7 Dunn et al., 2016 N/A Tg(elavl3:ReaChR-TagRFP-T)jf10 Dunn et al., 2016 N/A Tg(pet1:EGFP)ne0214Tg Lillesaar et al., 2009 N/A Tg(gad1b:loxP-RFP-loxP-GFP)nns26Tg Satou et al., 2013 N/A Tg(elavl3:GCaMP6f)jf1 Dunn et al., 2016 N/A Tg(tph2:epNTR-TagRFP)jf41 Ahrens lab; ZIRC N/A Software MATLAB R2014a Mathworks http://www.mathworks.com/products/matlab/ CUDA Nvidia Corporation https://developer.nvidia.com/cuda-toolkit SPM8 University College London www.fil.ion.ucl.ac.uk/spm/software/spm8/ CUDA script for Image registration Ahrens lab https://github.com/ahrens-lab/Kawashima_et_al_ Cell_2016/ MATLAB script for neuron detection Ahrens lab https://github.com/ahrens-lab/Kawashima_et_al_ Cell_2016/ MATLAB script for calculating brain deformation Ahrens lab https://github.com/ahrens-lab/Kawashima_et_al_ Cell_2016/ ..
Transgenic Assay:Article Title: The Serotonergic System Tracks the Outcomes of Actions to Mediate Short-Term Motor Learning.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-5-HT rabbit polyclonal antibody Sigma-Aldrich S5545; RRID: AB_477522 anti-rabbit secondary antibody conjugated with Cy3 Jackson ImmunoResearch 711-165-152; RRID: AB_2307443 anti-rabbit secondary antibody conjugated with Cy5 Jackson ImmunoResearch 711-175-152; RRID: AB_2340607 Alexa Fluor 594 hydrazide, sodium salt Thermo Fisher Scientific A10438 Chemicals for Pharmacology Assays, Paralysis, and Anesthesia Hoechst 33342 Life Technologies H3570 Alpha-Bungarotoxin Life Technologies B1601 Tricaine Sigma-Aldrich A5040 Para-chlorophenylalanine (pCPA) Tocris Bioscience 0938 Metronidazole MP Biomedicals 02155710 DNA Materials epNTR-TagRFP Tabor et al., 2014 N/A tph2 promoter in Tol2 vector Yokogawa et al., 2012 N/A Transgenic Animals Tg(elavl3:H2B-GCaMP6f)jf7 Dunn et al., 2016 N/A Tg(elavl3:ReaChR-TagRFP-T)jf10 Dunn et al., 2016 N/A Tg(pet1:EGFP)ne0214Tg Lillesaar et al., 2009 N/A Tg(gad1b:loxP-RFP-loxP-GFP)nns26Tg Satou et al., 2013 N/A Tg(elavl3:GCaMP6f)jf1 Dunn et al., 2016 N/A Tg(tph2:epNTR-TagRFP)jf41 Ahrens lab; ZIRC N/A Software MATLAB R2014a Mathworks http://www.mathworks.com/products/matlab/ CUDA Nvidia Corporation https://developer.nvidia.com/cuda-toolkit SPM8 University College London www.fil.ion.ucl.ac.uk/spm/software/spm8/ CUDA script for Image registration Ahrens lab https://github.com/ahrens-lab/Kawashima_et_al_ Cell_2016/ MATLAB script for neuron detection Ahrens lab https://github.com/ahrens-lab/Kawashima_et_al_ Cell_2016/ MATLAB script for calculating brain deformation Ahrens lab https://github.com/ahrens-lab/Kawashima_et_al_ Cell_2016/ ..
Software:Article Title: The Serotonergic System Tracks the Outcomes of Actions to Mediate Short-Term Motor Learning.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-5-HT rabbit polyclonal antibody Sigma-Aldrich S5545; RRID: AB_477522 anti-rabbit secondary antibody conjugated with Cy3 Jackson ImmunoResearch 711-165-152; RRID: AB_2307443 anti-rabbit secondary antibody conjugated with Cy5 Jackson ImmunoResearch 711-175-152; RRID: AB_2340607 Alexa Fluor 594 hydrazide, sodium salt Thermo Fisher Scientific A10438 Chemicals for Pharmacology Assays, Paralysis, and Anesthesia Hoechst 33342 Life Technologies H3570 Alpha-Bungarotoxin Life Technologies B1601 Tricaine Sigma-Aldrich A5040 Para-chlorophenylalanine (pCPA) Tocris Bioscience 0938 Metronidazole MP Biomedicals 02155710 DNA Materials epNTR-TagRFP Tabor et al., 2014 N/A tph2 promoter in Tol2 vector Yokogawa et al., 2012 N/A Transgenic Animals Tg(elavl3:H2B-GCaMP6f)jf7 Dunn et al., 2016 N/A Tg(elavl3:ReaChR-TagRFP-T)jf10 Dunn et al., 2016 N/A Tg(pet1:EGFP)ne0214Tg Lillesaar et al., 2009 N/A Tg(gad1b:loxP-RFP-loxP-GFP)nns26Tg Satou et al., 2013 N/A Tg(elavl3:GCaMP6f)jf1 Dunn et al., 2016 N/A Tg(tph2:epNTR-TagRFP)jf41 Ahrens lab; ZIRC N/A Software MATLAB R2014a Mathworks http://www.mathworks.com/products/matlab/ CUDA Nvidia Corporation https://developer.nvidia.com/cuda-toolkit SPM8 University College London www.fil.ion.ucl.ac.uk/spm/software/spm8/ CUDA script for Image registration Ahrens lab https://github.com/ahrens-lab/Kawashima_et_al_ Cell_2016/ MATLAB script for neuron detection Ahrens lab https://github.com/ahrens-lab/Kawashima_et_al_ Cell_2016/ MATLAB script for calculating brain deformation Ahrens lab https://github.com/ahrens-lab/Kawashima_et_al_ Cell_2016/ ..
other:Article Title: Adeno-associated viral tools to trace neural development and connectivity across amphibians.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EdU (5-ethynyl-2’-deoxyuridine) Invitrogen A10044 2-methylbutane Sigma-Aldrich M32631 KwikSil Silicone Adhesive World Precision Instruments KWIK-SIL Paraformaldehyde Electron microscopy sciences 15710 Methanol Acros organics 326950010 Hydrogen peroxide Sigma 216763-500ml SSCT 20X Fisher Scientific 15-557-044 Hybridization buffer Molecular Instruments N/A Wash buffer Molecular Instruments N/A Amplification buffer Molecular Instruments N/A DEPC (diethyl pyrocarbonate) Sigma-Aldrich D5758 DCM (dichloromethane) Sigma-Aldrich 34856 DAPI Sigma-Aldrich D9542-1MG Critical commercial assays Click-iT EdU Kit AF647 Invitrogen C10340 Experimental models: Organisms/strains Pleurodeles waltl Columbia University N/A Xenopus laevis, pigmented ISTA N/A Xenopus laevis, albino ISTA N/A Xenopus laevis, pigmented Xenopus Express N/A Pelophylax bedriagae Wild caught, Israel N/A Oligonucleotides PHP-CAG-GFP mFISH-HCR probes Integrated DNA Technologies This study; Table S4 Slc17A7 mFISH-HCR probes Integrated DNA Technologies This study; Table S4 HCR Amplifier B1-647 Molecular instruments N/A HCR Amplifier B2-488 Molecular instruments N/A Recombinant DNA CAG-EGFP Gift From Fred Gage, Salk Institute Addgene plasmid #16664 Software and algorithms Fiji 2.14.0 Schindelin et al.133 https://fiji.sc/ FactoMineR (RStudio 4.3.2) Lê et al.91 https://CRAN.R-project.org/ package=FactoMineR Prism 10 Graphpad Software, Inc. https://www.graphpad.com/ features ZenBlue 3.7 Carl Zeiss Microscopy N/A Imaris 9.9.1 Oxford Instruments https://imaris.oxinst.com/ Other Nanoject III Programmable Injector Drummond 3-000-207 PicoSpritzer injection setup World Precision Instruments PV820 Electric capillary beveler Narishige EG-400 Automatic capillary puller Sutter Instrument P-97 Glass capillaries Drummond 3-000-203-G/X 18 Well Chamber Slides Ibidi 81816 Nylon suture kit size 5-0 Ethicon V2130H
Article Title: Sodium channel Na V 1.3 is important for enterochromaffin cell excitability and serotonin release
Article Snippet: Human Colon Source Titer Secondary Source Titer NaV1.3 Alomone (ACS004) Colon: 1:100 (10 μg/mL) Small bowel: 1:400 (2.5 μg/mL) Cy3 Jackson ImmunoReseach (111-165-003) 1:800 (1.25 μg/mL) 5-HT Abcam (ab66047) 1:2000 (0.25 μg/mL) Cy5 Jackson ImmunoResearch (705-175-147) 1:800 (1.5 μg/mL) Mouse Colon and Small bowel Source Titer Secondary Source Titer NaV1.3 Alomone (ACS-004) 1:100 (10 μg/mL) Cy3 Jackson ImmunoReseach (111-165-003) 1:800 (1.25 μg/mL) Supplementary Table 2.
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